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KMID : 0191120080230030492
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Journal of Korean Medical Science
2008 Volume.23 No. 3 p.492 ~ p.501
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The Role of Cyclosporine and Mycophenolate in an Orthotopic Porcine-to-Rat Corneal Xenotransplantation
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Lee Hyeon-IL
Kim Mee-Kum
Oh Joo-Youn
Ko Jung-Hwa
Lee Hyun-Ju
Wee Won-Ryang
Lee Jin-Hak
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Abstract
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We performed this study to investigate the feature of rejection in porcine-to-rat corneal
orthotopic transplantation and to evaluate the effect of cyclosporine and mycophenolate
on the xeno-rejection. Orthotopic corneal transplantation was done at 91
Sprague-Dawley rats, and they were divided into 10 groups based on the combination
of immunosuppressants including dexamethasone, cyclosporine, and mycophenolate
mofetil. Graft survival was analyzed and grafted eyes were examined with
Hematoxylin & Eosin and CD4 or CD8 staining. Enzyme-linked immunosorbent
assays were done for interleukin-2 (IL-2), IL-4, IL-5, IL-10, and interferon (IFN)-γin
cornea, lacrimal gland, and cervical lymph nodes. The longest median survival of
the immune suppressant group was 11.00±1.96 days, which showed no statistical
differences compared with that of control (8.00±1.52 days). The neutrophils
were prominent in the early phase but soon gave way to the monocytes. The number
of CD8+ cells was higher than that of CD4+ cells. IL-2 and IFN-γmarkedly
increased at 10 to13 days in cornea, lacrimal glands, and cervical lymph nodes,
which showed a decrease with immunosuppressants except in the cornea. In conclusion,
cyclosporine and mycophenolate could not prevent the rejection in porcine
to rat orthotopic corneal xenograft associated with infiltraton of CD8+ and innate
immune cells.
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KEYWORD
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Cornea, Transplantation, Heterologous, Immunosuppression, Rats, Swine
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